Spectrophotometric determination of 0–50 ng of chromium in 1 ml of human serum

Abstract

A method is described for the determination of chromium in 1 ml of human serum or plasma. It is based on a wet decomposition and a spectrophotometric determination with diphenylcarbazide after extraction with methyl isobutyl ketone. A 40-mm cuvette with less than 1 ml sample volume is described. Results are lower than expected from the literature, most samples from healthy persons containing less than 2 or 3 ng/ml. The reproducibility is about 1 ng/ml.