Abstract
1. 1. Human erythrocyte phosphofructokinase (ATP:-fructose 6-phosphate 1-phosphotransferase, EC 2.7.1.11) was purified 15 000-fold by (NH4)2SO4 precipitation, heating and column chromatography on Sepharose 6-B. The resulting enzyme preparation had a specific activity of 60 μmoles Fru-1,6-P2 formed per min per mg protein at 25 °C.
2. 2. With cellulose-acetate electrophoresis only
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