Abstract
Prolonged culturing of rodent cells in vitro activates
p19ARF (named p14ARF in man), resulting in a p53-dependent
proliferation arrest known as senescence. The
p19ARF-Mdm2-p53 pathway also serves to protect primary
cells against oncogenic transformation. We have
used a genetic screen in mouse neuronal cells, conditionally
immortalized by a temperature-sensitive mutant
of SV40 large T antigen, to identify
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