Arthrobacter luteus restriction endonuclease cleavage map of X174 RF DNA

Abstract

Cleavage of X174 RF DNA with the restriction endonuclease from Arthrobacter luteus (Alu I) produces 23 fragments of approximately 24–1100 base pairs in length. The order of most of these fragments has been established by digestion of Haemophilus influenzae Rd (Hind II) and Haemophilus aegyptius (Hae III) endonuclease fragments of X RF with Alu I and by reciprocal digestions of Alu I fragments with Hind II and Hae III. In this way the Arthrobacter luteus map could be aligned with the Hind II and Hae III cleavage maps of X174 RF DNA of A. S. Lee and R. L. Sinsheimer ((1974) Proc. Nat. Acad. Sci. USA 71, 2882–2886).