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Janine C. Deddens, Dries A. Feyen, Peter-Paul Zwetsloot, Maike A. Brans, Sailay Siddiqi, Linda W. van Laake, Pieter A. Doevendans, Joost P. Sluijter
Targeting chronic cardiac remodeling with cardiac progenitor cells in a murine model of ischemia/reperfusion injury
www.plosone.org
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11.9999 0 0 11.9999 200.0125 622.9417 cm
BT
/F0 1 Tf
1 TL
-0.0033 Tc
0 0 Td
(CPC)Tj
2.3102 0 Td
(isolation,)Tj
4.1103 0 Td
(expansion)Tj
4.7197 0 Td
(and)Tj
1.8614 0 Td
(transduction)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 605.9338 cm
BT
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1 TL
-0.004 Tc
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3.3165 0 Td
(fetal)Tj
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(heart)Tj
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(permission)Tj
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(standard)Tj
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(consent)Tj
3.3279 0 Td
(procedures)Tj
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(and)Tj
1.7348 0 Td
(prior)Tj
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(approval)Tj
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(of)Tj
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(the)Tj
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(ethics)Tj
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(committee)Tj
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(of)Tj
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(Utrecht,)Tj
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(the)Tj
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(Netherlands.)Tj
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1.9899 0 Td
(procedure)Tj
4.3086 0 Td
(is)Tj
0.8504 0 Td
(in)Tj
1.0375 0 Td
(accordance)Tj
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(with)Tj
1.9956 0 Td
(the)Tj
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(principles)Tj
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(outlined)Tj
3.5659 0 Td
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(the)Tj
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(of)Tj
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(Helsinki)Tj
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(human)Tj
3.0784 0 Td
(tissue)Tj
2.4605 0 Td
(or)Tj
1.0998 0 Td
(subjects.)Tj
3.6 0 Td
(CPC)Tj
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q
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1 J
0 w
7.4999 0 0 6.6659 278.8157 558.3685 cm
BT
/F7 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(+)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 285.3354 553.9464 cm
BT
/F7 1 Tf
1 TL
-0.004 Tc
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(conjugated)Tj
4.6317 0 Td
(magnetic)Tj
3.9005 0 Td
(beads)Tj
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(described)Tj
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(previously)Tj
4.3483 0 Td
([)Tj
0.83 0.64 0.02 0 k
(22)Tj
0 g
(].)Tj
2.0806 0 Td
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(CPC)Tj
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(able)Tj
1.8312 0 Td
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1.8368 0 Td
(cardiomyocytes,)Tj
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(endothelial)Tj
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(their)Tj
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(stemness)Tj
3.821 0 Td
([)Tj
0.83 0.64 0.02 0 k
(23)Tj
0 g
(].)Tj
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1.338 0 Td
(facilitate)Tj
3.5999 0 Td
(identification)Tj
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5.5843 0 Td
[()12()]TJ
1.0148 0 Td
[()-13()15()3()]TJ
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(,)Tj
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(a)Tj
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4.524 0 Td
(pLV-CMV-luc-GFP)Tj
8.2714 0 Td
(as)Tj
1.0148 0 Td
(described)Tj
4.0536 0 Td
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(24)Tj
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(SP++)Tj
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(\(M199,)Tj
3.1066 0 Td
(EGM2,)Tj
3.1011 0 Td
(FBS,)Tj
2.0239 0 Td
(P/S,)Tj
1.8142 0 Td
(NEAA\))Tj
3.2315 0 Td
(until)Tj
2.1089 0 Td
(80%)Tj
1.9276 0 Td
(confluency)Tj
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(and)Tj
1.7348 0 Td
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1.389 0 Td
[()12()]TJ
1.0148 0 Td
[()-13()15()3()]TJ
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-35.1323 -1.2983 Td
(transplantation)Tj
6.3326 0 Td
(at)Tj
0.9524 0 Td
(passage)Tj
3.2144 0 Td
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ET
Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 456.4346 cm
BT
/F0 1 Tf
1 TL
-0.0033 Tc
0 0 Td
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ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 439.4267 cm
BT
/F7 1 Tf
1 TL
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1.3946 0 Td
(experiments)Tj
5.142 0 Td
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(out)Tj
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(in)Tj
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(accordance)Tj
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(with)Tj
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(the)Tj
1.474 0 Td
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1.3833 0 Td
(the)Tj
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(of)Tj
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-34.6221 -1.2982 Td
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2.6532 0 Td
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(with)Tj
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(prior)Tj
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(approval)Tj
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1.1792 0 Td
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2.9877 0 Td
(Experimentation)Tj
6.9789 0 Td
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(Utrecht)Tj
3.2995 0 Td
(University,)Tj
4.6261 0 Td
(the)Tj
1.4683 0 Td
(Netherlands.)Tj
5.3461 0 Td
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1.2643 0 Td
(CPC)Tj
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1.0147 0 Td
(human)Tj
3.0785 0 Td
(origin)Tj
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(immune)Tj
3.6283 0 Td
(compro-)Tj
-33.9758 -1.3039 Td
(mised)Tj
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(mice)Tj
2.1544 0 Td
(\(NOD-SCID)Tj
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(mice,)Tj
2.3811 0 Td
(Harlan)Tj
3.0217 0 Td
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1.0261 0 Td
(prevent)Tj
3.2541 0 Td
(graft)Tj
2.0807 0 Td
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Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 367.937 cm
BT
/F0 1 Tf
1 TL
-0.0033 Tc
0 0 Td
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4.1764 0 Td
(reperfusion)Tj
5.1591 0 Td
(model)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 350.9858 cm
BT
/F7 1 Tf
1 TL
-0.004 Tc
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2.2166 0 Td
(NOD-SCID)Tj
5.006 0 Td
(mice,)Tj
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(aged)Tj
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(10-12)Tj
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(left)Tj
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(coronary)Tj
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(artery)Tj
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(\(LAD\))Tj
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(ligation)Tj
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(described)Tj
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0.83 0.64 0.02 0 k
(25)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
1.7348 0 Td
(26)Tj
0 g
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1.7348 0 Td
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(by)Tj
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(reperfusion)Tj
4.8245 0 Td
(after)Tj
2.0296 0 Td
(60)Tj
1.1679 0 Td
(minutes)Tj
3.4639 0 Td
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3.7588 0 Td
(the)Tj
1.4626 0 Td
(ligature)Tj
-34.5597 -1.2982 Td
(and)Tj
1.7291 0 Td
(removal)Tj
3.4752 0 Td
(of)Tj
1.0148 0 Td
(tubing.)Tj
3.0614 0 Td
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2.9877 0 Td
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(confirmed)Tj
4.3937 0 Td
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1.1848 0 Td
(reversed)Tj
3.5773 0 Td
(discoloration)Tj
5.4822 0 Td
(of)Tj
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(the)Tj
1.474 0 Td
(heart.)Tj
ET
Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 292.4787 cm
BT
/F0 1 Tf
1 TL
-0.0033 Tc
0 0 Td
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1.9134 0 Td
(transplantation)Tj
6.6945 0 Td
(model)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 275.4708 cm
BT
/F7 1 Tf
1 TL
-0.004 Tc
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5.5728 0 Td
(days)Tj
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2.0353 0 Td
(induction)Tj
4.1385 0 Td
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(myocardial)Tj
4.6998 0 Td
(injury,)Tj
2.8743 0 Td
(animals)Tj
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(injected)Tj
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1.9956 0 Td
(either)Tj
-33.1708 -1.2982 Td
(CPC,)Tj
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(or)Tj
1.0942 0 Td
(vehicle)Tj
2.982 0 Td
(\(PBS\))Tj
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(\()Tj
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(Fig)Tj
1.8142 0 Td
(1)Tj
0 g
(\).)Tj
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(determine)Tj
4.3143 0 Td
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(extent)Tj
2.6702 0 Td
(of)Tj
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(injury,)Tj
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(mice)Tj
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(underwent)Tj
4.5694 0 Td
(echocardiog-)Tj
-32.3657 -1.2983 Td
(raphy)Tj
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(\(echo\))Tj
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1.1792 0 Td
(randomization)Tj
6.1625 0 Td
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3.6566 0 Td
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(an)Tj
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(parasternal)Tj
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(long)Tj
1.9842 0 Td
(axis)Tj
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(view)Tj
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(\(PSLAX\))Tj
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1.3833 0 Td
(intramyocardial)Tj
6.6273 0 Td
(injection.)Tj
3.9855 0 Td
(CPC)Tj
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(or)Tj
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(vehicle)Tj
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3.3902 0 Td
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1.0374 0 Td
(the)Tj
1.4684 0 Td
(anterior)Tj
3.4412 0 Td
(wall)Tj
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(a)Tj
0.6577 0 Td
(transthoracic)Tj
5.4821 0 Td
(approach)Tj
3.9515 0 Td
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(echo)Tj
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(27)Tj
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(5)Tj
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0.6973 0 Td
[()]TJ
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(l)Tj
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(targeting)Tj
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(the)Tj
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(same)Tj
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(`bor-)Tj
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ET
Q
q
1 j
1 J
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BT
/F0 1 Tf
1 TL
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(imaging)Tj
3.6709 0 Td
(\(BLI\))Tj
ET
Q
q
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1 J
0 w
10 0 0 10 200.0125 148.0251 cm
BT
/F7 1 Tf
1 TL
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(determine)Tj
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1.474 0 Td
(amount)Tj
3.3505 0 Td
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(emitted)Tj
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-35.121 -1.3039 Td
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(24)Tj
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0 Tc
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-0.004 Tc
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(20.000)Tj
2.8232 0 Td
(ph/s/cm)Tj
ET
Q
q
1 j
1 J
0 w
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BT
/F7 1 Tf
1 TL
-0.0053 Tc
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(2)Tj
ET
Q
q
1 j
1 J
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BT
/F7 1 Tf
1 TL
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(established)Tj
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(24)Tj
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(analyses,)Tj
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(a)Tj
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(low)Tj
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(<)Tj
/F7 1 Tf
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1.3323 0 Td
(20.000)Tj
2.8233 0 Td
(ph/s/cm)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 274.6204 87.4204 cm
BT
/F7 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(2)Tj
ET
Q
q
1 j
1 J
0 w
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BT
/F7 1 Tf
1 TL
-0.004 Tc
0 0 Td
(/sr\))Tj
1.6214 0 Td
(were)Tj
2.1259 0 Td
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(for)Tj
1.3833 0 Td
(additional)Tj
4.2803 0 Td
(analyses.)Tj
ET
Q
q
1 j
1 J
0 w
576 737.1 m
36 737.1 l
36 737.6 l
576 737.6 l
f*
36 741.2598 107.1496 23.6976 re
W* n
q
107.0929 0 0 23.6409 36 741.3165 cm
q
/I0 Do
Q
Q
Q
q
0 0 612 792 re
W* n
1 j
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