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Gene expression and apoptosis in bovine embryos during in vitro culture and in vivo development Gene expression and apoptosis in bovine embryos during in vitro culture and in vivo development / Hiemke Maria Knijn - [S.l.] : [s.n.], 2004 - Tekst. - Proefschrift Universiteit Utrecht |
NBC: 46.60 : diergeneeskunde: voortplanting
Trefwoorden: blastocyst, maturation, culture, in vitro, in vivo, gene expression, apoptosis, embryo quality
The first attempts to fertilise in vitro bovine oocytes were done in the late sixties but only in 1982 the first calf was born after transplantation of a complete in vitro produced embryo. Since then the in vitro production system improved a lot but it is still impossible to mimic the in vivo situation completely. Not only the efficiency of the in vitro procedure to produce morulae and blastocysts is lower then in the in vivo situation but also the quality of the in vitro produced morulae and blastocysts is lower compared to in vivo developed counterparts. The pregnancy rate after transfer of in vitro produced embryos is in general lower compared to in vivo developed embryos and a small but significant higher percentage of calves are born with abnormalities known as the "Large Offspring Syndrome". |