Chicken mannose binding lectin has antiviral activity towards infectious bronchitis virus
Zhang, Weidong; Bouwman, Kim M; van Beurden, Steven J; Ordonez, Soledad R; van Eijk, Martin; Haagsman, Henk P; Verheije, M Hélène; Veldhuizen, Edwin J A
(2017) Virology, volume 509, pp. 252 - 259
(Article)
Abstract
Mannose binding lectin (MBL) is a collagenous C-type lectin, which plays an important role in innate immunity. It can bind to carbohydrates on the surface of a wide range of pathogens, including viruses. Here we studied the antiviral effect of recombinant chicken (rc)MBL against Infectious Bronchitis Virus (IBV), a highly
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contagious coronavirus of chicken. rcMBL inhibited in a dose-dependent manner the infection of BHK-21 cells by IBV-Beaudette, as detected by immunofluorescence staining of viral proteins and qPCR. ELISA and negative staining electron microscopy showed that rcMBL bound directly to IBV, resulting in the aggregation of viral particles. Furthermore, we demonstrated that MBL bound specifically to the spike S1 protein of IBV which mediates viral attachment. This subsequently blocked the attachment of S1 to IBV-susceptible cells in chicken tracheal tissues as shown in protein histochemistry. Taken together, rcMBL exhibits antiviral activity against IBV, based on a direct interaction with IBV virions.
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Keywords: Animals, Antiviral Agents, Cell Line, Chickens, Cricetinae, Enzyme-Linked Immunosorbent Assay, Fluorescent Antibody Technique, Immunity, Innate, Infectious bronchitis virus, Mannose-Binding Lectin, Microscopy, Electron, Transmission, Protein Binding, Real-Time Polymerase Chain Reaction, Spike Glycoprotein, Coronavirus, Virion, Virus Attachment, Journal Article
ISSN: 0042-6822
Publisher: Academic Press Inc.
Note: Copyright © 2017 The Authors. Published by Elsevier Inc. All rights reserved.
(Peer reviewed)
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