Measuring Anisotropy in Fluorescence Spectroscopy Experiments - From Data to Information

Abstract

New methods to analyze the anisotropy in spectral images of fluorophores are presented. These methods expand on the recent development of the phasor analysis concerning the unmixing of spectral images. The methods introduced solve for the anisotropy via a minimization method as well via an enhancement on already developed theory. Furthermore an algorithm is developed which finds anisotropy based on linear unmixing methods. Results are shown for simulated data as well as real measured data.