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[(\))-247.1(and)-239.8(plated)-248(in)-240.9(noncoated)-248.4(25)-169.7(cm)]TJ
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[(\(Life)-431(Technologies\),)-327.8(100)]TJ
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[(mL)-430.5(streptomycin)-432.5(\(Life)-431(Technol-)]TJ
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[(mL)-272.4(heparin)-277.3(\(LEO)-268.8(Pharma\))-274.4(as)-272.7(previously)]TJ
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T*
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[(Ca)272(t5)272(%)5.3(C)272(O)]TJ
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[(in)-266.2(a)-271.4(humid)-268.1(atmosphere.)-275.4(The)-271.9(medium)-268.5(was)]TJ
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T*
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T*
[(phosphate-buffered)-201.2(saline)-188.2(\(UMC)-190.5(Pharmacy\))-189.6(containing)-188.1(2)-163.9(m)0(M)]TJ
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[(ethylenediaminetetraacetic)-641.7(acid)-631.3(and)-631.9(then)-633.1(incubated)-634.5(with)]TJ
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[(trypsinethylenediaminetetraacetic)-428.7(acid)-410(\(Lonza\).)-410.4(The)-417.3(MSCs)]TJ
T*
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T*
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[(CFU)-10(-F)-333.4(as)-6.4(say)]TJ
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[(To)-290.3(determine)-292.9(the)-288.3(CFU-F)-289.1(frequency)-292(in)-285.1(BM)-292.6(or)-288(uorescence-)]TJ
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[(activated)-436.4(cell)-429.9(sorter)-435.8(\(FACS\)enriched)-435.3(MNCs,)-433.1(graded)-427.8(num-)]TJ
T*
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[(Falcon)-278.5(T-25)-280.7(asks)-275.1(with)-280.9(vented)-279.3(caps.)-276.3(The)-278.2(culture)-278.5(medium)-274.8(for)]TJ
0 -1.2203 TD
[(each)-432(species)-430.2(was)-426.1(used)-422.9(as)-430.8(described.)-422.6(After)-432.9(10)-422.7(days)-425.6(the)-427.4(ad-)]TJ
T*
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[(saline)-536(and)-537(subsequently)-531.6(xed)-532.9(with)-540.1(ice-cold)-530.3(methanol)-535.3(for)]TJ
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[(15)-169.7(min)-282.7(at)-284.5(4)]TJ
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(8)Tj
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[(C.)-287.6(To)-284(observe)-287.9(the)-282(colonies,)-291.3(the)-282(cells)-285.8(were)-286.7(stained)]TJ
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[(with)-672.9(Giemsa)-670.8(\(Sigma-Aldrich\))-677.3(diluted)-666(1:8)-672.2(with)-666.6(H)]TJ
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(2)Tj
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[(O)-667(for)]TJ
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[(15)-169.7(min)-447.1(at)-448.9(room)-446.4(temperature)-450.8(and)-442.2(washed)-451.3(twice)-447.4(with)-445.3(H)]TJ
6.7246 0 0 5.9768 539.603 400.9889 Tm
(2)Tj
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(O.)Tj
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[(CFU-F)-289.1(colonies)-294.7(containing)-289.2(at)-290.8(least)-289.3(50)-163.4(cells)-292.1(were)-286.7(scored)-291.5(using)]TJ
T*
[(a)-334.6(Stereomicroscope)-339.5(\(Zeiss\).)]TJ
/F9 1 Tf
9.9626 0 0 9.9626 313.0582 360.0566 Tm
[(Ant)-9(ibodies,)-342.1(ow)-335.5(cytome)-8.5(tric)-335.3(analysi)-9.3(s,)-334.3(and)-335.7(sorting)]TJ
/F3 1 Tf
8.9663 0 0 8.9663 323.0361 342.1983 Tm
[(For)-380.9(the)-383.1(initial)-377.9(screening)-382.5(of)-376.3(cultured)-382.6(MSCs)-384.5(from)-380.9(the)-376.8(non-)]TJ
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[(human)-409.9(species,)-414.2(we)-406.2(used)-410.2(a)-410.5(panel)-412.4(of)-407.9(43)-163.4(mAb)-408(that)-414.8(contained)]TJ
T*
[(the)-471.7(following)-468.7(commercially)-466.8(available)-469.4(mAb)-465(against)-467.4(human)]TJ
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[(CD3,)-313.8(13,)-318.1(14,)-311.8(19,)-311.8(29,)-311.8(31,)-311.8(34,)-311.8(44,)-311.8(45,)-311.8(49,)-318.1(55,)-311.8(71,)-311.8(73,)-311.8(90,)-311.8(117,)-311.3(146,)]TJ
0 -1.2203 TD
[(HLA-ABC,)-410.7(HLA-DR,)-411.7(Sca-1)-408.2([45])-404.6(\(Becton)-407.6(Dickinson\),)-412.1(CD105)]TJ
T*
[(\(Ancell)-428.8(Corp.\),)-428.6(CD235a)-429.6(\(Dako\),)-422.9(CD271)-430.1(\(Clone)-427.6(20.4)-425.1(against)]TJ
0 -1.2266 TD
[(low-afnity)-497.4(nerve)-491.5(growth)-493.9(factor)-496.4(receptor-allophycocyanin;)]TJ
0 -1.2203 TD
[(Miltenyi)-238.9(Biotec\),)-240(ALP)-233.4(\(clone)-237(B4-78-c;)-238.6(Hybridoma)-237.2(Bank\),)-235(KDR)]TJ
T*
[(\(R&D)-797.9(Systems\),)-803.5(SSEA-4)-798(\(clone)-793.4(MC-813-70;)-797.1(Hybridoma)]TJ
T*
[(Bank\),)-614.4(W8B2)-608(against)-612.8(human)-612.2(MSCs)-612.1(antigen-1)-615.3(\()-82.7(MSCA-1)]TJ
/F7 1 Tf
26.1705 0 TD
(=)Tj
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[(W8B2-phycoerythrin)-351.7(\(PE\);)-343.3(Biolegend\),)-349.3(W4A5)-340.9(against)-347.3(MSCs,)]TJ
0 -1.2203 TD
[(and)-695.1(neural)-697.6(progenitor)-696.6(cells)-690.4(\(W4A5-PE;)-696.9(Biolegend\).)-697.1(Ad-)]TJ
T*
[(ditionally,)-397.3(we)-399.9(used)-391.3(a)-397.9(series)-392.3(of)-401.6(mAb)-389.1(that)-395.9(showed)-397.7(reactivity)]TJ
0 -1.2267 TD
[(with)-293.6(the)-288.3(CD271)]TJ
/F4 1 Tf
6.7246 0 0 5.9768 374.2298 203.7543 Tm
()Tj
/F3 1 Tf
8.9663 0 0 8.9663 381.9967 199.729 Tm
[(subpopulation)-292.7(in)-285.1(human)-289.7(BM)-286.3(with)-293.6(putative)]TJ
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[(MSCs)-510.9(specicity)-509.3([46].)-508.7(These)-513.3(mAb)-509.2(consisted)-508.2(of)-509.1(the)-509.6(clones)]TJ
T*
[(W3C4)-390.2(\(CD349;)-382.9(frizzled-9\),)-386.6(W5C4,)-386.8(and)-385.3(39D5)-385.6(\(CD56)-386.8(epitope)]TJ
0 -1.2266 TD
[(expressed)-275.1(on)-265.4(MSCs)-264.3(but)-265.5(not)-265.9(on)-265.4(NK)-268.4(cells\))-267.3(or)-269(MY3)111.3(1)-265.1(\(anti-CD56)]TJ
0 -1.2203 TD
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T*
[(\(CD340\),)-396(W5C5,)-386.8(58B)105.5(1,)-388.1(CH3A4A7A)-394.9(\(CD344\),)-389.7(67D2)-392(\(CD)111.8(164\),)]TJ
0 -1.2266 TD
[(W7C5)-497.7(\(CD)105.5(109\),)-495.1(67A4)-493.1(\(CD324\),)-503.5(28D4)-493.1(\(CD)105.5(140b\),)-493.2(HEK-3D6,)]TJ
0 -1.2203 TD
[(W3D5,)-267.9(and)-265.1(CUB)109.2(1)-265.1(\(CD3)105.9(18\))-265.1([4)113.8(143].)-267(Binding)-270.9(of)-268.8(nonconjugated)]TJ
T*
[(mAb)-458.6(was)-451.4(analyzed)-455.4(by)-451.4(staining)-455.8(with)-457.9(isotype-specic)-458.2(\(IgG,)]TJ
T*
[(IgG)108.5(1,)-331.2(IgM,)-330.7(or)-332.3(IgG3\))-334.1(PE-conjugated)-333.7(goat)-329.6(anti-mouse)-339.1(antisera)]TJ
0 -1.2266 TD
[(\(Southern)-466.2(Biotechnology)-457.3(Associates,)-462.7(Inc.\).)-453.7(Flow)-461.3(cytometric)]TJ
0 -1.2203 TD
[(analysis)-320(was)-312.3(performed)-316.2(on)-316(a)-309.3(FACS)-314.3(Calibur,)-319.6(and)-309.4(cell)-316.1(sorting)]TJ
T*
[(was)-306(performed)-303.5(on)-297(a)-303(FACSAria)-300.5(cytometer)-302.7(\(Becton)-306.5(Dickinson)]TJ
0 -1.2267 TD
[(Biosciences\).)-267.2(About)-149.5(1)]TJ
/F4 1 Tf
9.1872 0 TD
()Tj
/F3 1 Tf
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(10)Tj
6.7246 0 0 5.9768 409.3227 61.285 Tm
(6)Tj
8.9663 0 0 8.9663 414.992 57.2598 Tm
[(primary)-260.7(BM)-261(cells)-260.5(or)-148.9(1)]TJ
/F4 1 Tf
9.3516 0 TD
()Tj
/F3 1 Tf
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(10)Tj
6.7246 0 0 5.9768 513.4676 61.285 Tm
(4)Tj
8.9663 0 0 8.9663 519.1369 57.2598 Tm
(cultured)Tj
/F5 1 Tf
-50.9816 77.2217 TD
(1912)Tj
44.1592 0 TD
[(ROZEMULLER)-335.7(ET)-338.7(AL.)]TJ
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